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Chylomicron apoprotein alteration after plasma exposure.

S F Robinson, S H Quarfordt

    Biochimica Et Biophysica Acta
    |July 17, 1978
    PubMed
    Summary

    Chylomicrons incubated with rat plasma show significant changes in apoproteins, particularly an increase in arginine-rich apoprotein and a decrease in Apo A-I. These alterations occur physiologically during alimentary lipemia.

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    Area of Science:

    • Lipid metabolism
    • Apolipoprotein research
    • Biochemistry

    Background:

    • Chylomicrons are key carriers of dietary lipids.
    • Understanding chylomicron apoprotein dynamics is crucial for lipid metabolism research.

    Purpose of the Study:

    • To investigate changes in chylomicron lipid and apoprotein constituents upon incubation with rat plasma.
    • To determine the physiological relevance of observed apoprotein alterations in alimentary lipemia.

    Main Methods:

    • Incubation of purified rat lymph chylomicrons with chylomicron-free rat plasma or HDL fraction.
    • Analysis of apoprotein and lipid constituents.
    • Heparin affinity chromatography to assess changes in affinity.
    • Investigation of Lecithin:Cholesterol Acyl Transferase (LCAT) reaction impact.

    Main Results:

    • A five-fold increase in arginine-rich apoprotein and a reduction of Apo A-I were observed.
    • These changes were replicated by incubation with the HDL fraction.
    • Arginine-rich apoprotein associated with chylomicrons without lipid changes.
    • Increased apoprotein content correlated with enhanced heparin affinity.
    • Alterations were independent of triglyceride concentration and LCAT inhibition.

    Conclusions:

    • In vitro incubation of chylomicrons with plasma mimics in vivo apoprotein changes.
    • Observed apoprotein alterations likely occur physiologically in alimentary lipemia.
    • These findings contribute to understanding chylomicron metabolism and lipoprotein interactions.

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