Glycosylation of 3, 5, 3'-L-triiodothyronine and its biological activity

The formation of glycosylated Hb by the nonenzymatic reaction between Hb and glucose suggests that glycosylation is a general reaction in the living body, we demonstrated by means of TLC and HPLC that T3 was glycosylated when reacted with glucose in absolute ethanol for a week. We demonstrated in a metamorphic study in tadpoles that the biological activity of thus glycosylated T3 was less than about 1/10 of that of T3. These findings suggest that glycosylation of substances with amino group other than serum proteins including Hb may occur and that glycosylated substances may differ in biological activities from the original substances.