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[Poly A-protein complexes in polyribosomes of sprouting wheat embryos]
Biokhimiia (Moscow, Russia)
|June 1, 1978
Summary
Researchers isolated polyribosomes from wheat embryos. They found that poly-A sequences exist as protein complexes, not just RNA, in these polyribosomes.
Area of Science:
- Molecular Biology
- Plant Science
- Biochemistry
Context:
- Investigating the structure and function of polyribosomes in eukaryotic gene expression.
- Focusing on the role of polyadenylation in messenger RNA (mRNA) stability and translation.
- Examining the specific molecular components associated with poly-A tails in plant systems.
Purpose:
- To isolate and characterize polyribosomes from sprouting wheat embryos.
- To determine the nature of poly-A sequences within these polyribosomes.
- To investigate whether poly-A sequences are associated with proteins.
Summary:
- Purified polyribosomes were obtained from sprouting wheat embryos.
- Treatment with pancreatic RNase revealed poly-A sequences in 8-14 S fractions.
- These poly-A containing complexes exhibited characteristics of protein association, including membrane filter binding and pronase sensitivity.
Impact:
- Provides evidence for the existence of poly-A-protein complexes in wheat embryo polyribosomes.
- Contributes to understanding post-transcriptional gene regulation in plants.
- Highlights the structural organization of mRNA in translationally active complexes.