Cultivation of rat cerebellar cells under conditions of optimal oxygen supply

A method is described for the cultivation of essentially small cerebellar neurones under optimal oxygen supply. Cerebellar cells were seeded onto polylysine-coated dishes equipped with a gas-permeable bottom (Petriperm). Under these conditions, cells migrated to form small groups and developed dense networks of fibres covering the entire bottom of the dish. Contamination with non-neuronal cells was restricted to fibroblasts (less than 0.1%), oligodendrocytes (less than 1%) and astrocytes (approximately 6%), even after prolonged cultivation (15 days).