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Related Experiment Videos

Vimentin gene expression during myogenesis: two functional transcripts from a single copy gene.

Z E Zehner, B M Paterson

    Nucleic Acids Research
    |December 10, 1983
    PubMed
    Summary

    Chicken vimentin gene expression produces two functional mRNA transcripts. These transcripts are utilized identically across all tissues, with expression levels decreasing from embryonic to adult stages.

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    Area of Science:

    • Molecular Biology
    • Genomics
    • Cell Biology

    Background:

    • A single vimentin gene exists in the chicken genome.
    • In vivo transcription generates two distinct mRNA classes (approx. 2200 and 2500 nucleotides).
    • Differential utilization of polyadenylylation sites is suspected for mRNA generation.

    Purpose of the Study:

    • To confirm the functionality of both vimentin mRNA transcripts in cell-free synthesis.
    • To investigate the in vivo utilization of polyadenylylation signals within the vimentin gene.
    • To analyze the tissue-specific and developmental expression patterns of vimentin mRNA.

    Main Methods:

    • Cell-free synthesis and two-dimensional gel electrophoresis to assess polypeptide production.
    • S1 nuclease analysis to determine polyadenylylation site usage in vivo.

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  • Comparative analysis of vimentin mRNA levels across different chicken tissues and developmental stages.

    • Main Results:

    • Both vimentin mRNA transcripts are functional in vitro, directing cell-free synthesis of the vimentin polypeptide.
    • Three out of four potential polyadenylylation signals are utilized in vivo, identically across all examined chicken tissues.
    • The polyadenylylation site nearest the message body appears unused under the employed S1 analysis conditions.
    • Vimentin mRNA levels decrease during the embryonic to adult transition in all tissues.

    Conclusions:

    • The two vimentin mRNA transcripts are functional and their polyadenylylation site usage is conserved across chicken tissues.
    • Vimentin gene expression is not regulated by tissue-specific or developmental patterns, but rather by overall expression levels.
    • The observed decrease in vimentin mRNA levels during development suggests a role in cellular differentiation or tissue maturation.