Related Experiment Video

Updated: Aug 15, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Rate enhancement by guided diffusion. Chain length dependence of repressor-operator association rates

R Schranner, P H Richter

    Biophysical Chemistry
    |May 1, 1978
    PubMed

    More Related Videos

    An Assay for Quantifying Protein-RNA Binding in Bacteria07:02

    An Assay for Quantifying Protein-RNA Binding in Bacteria

    Published on: June 12, 2019

    Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution10:27

    Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

    Published on: July 8, 2019

    Related Concept Videos

    RNA Polymerase II Accessory Proteins02:36

    RNA Polymerase II Accessory Proteins

    Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
    Cooperative Binding of Transcription Regulators02:13

    Cooperative Binding of Transcription Regulators

    Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
    Prokaryotic Transcriptional Activators and Repressors01:58

    Prokaryotic Transcriptional Activators and Repressors

    The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
    Transcription of prokaryotic...
    Cooperative Binding of Transcription Regulators02:13

    Cooperative Binding of Transcription Regulators

    Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
    Operon Model01:23

    Operon Model

    The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
    Repressible Operon: trp Operon01:21

    Repressible Operon: trp Operon

    The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...