Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Respiratory pathways in Hansenula saturnus.

A M Viola, F Tassi, P Goffrini

Antonie Van Leeuwenhoek

|December 1, 1983

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rivers under stress: geochemical changes along a rural-urban gradient during extreme hydrological events (Greve River, Italy).

Environmental geochemistry and health·2026

Same author

Traditional and low-cost technical approaches for investigating greenhouse gases and particulate matter distribution along an urban-to-rural transect (Greve River Basin, Central Italy).

Environmental geochemistry and health·2025

Same author

Development and machine learning-based calibration of low-cost multiparametric stations for the measurement of CO<sub>2</sub> and CH<sub>4</sub> in air.

Heliyon·2024

Same author

Soil processes modify the composition of volatile organic compounds (VOCs) from CO<sub>2</sub>- and CH<sub>4</sub>-dominated geogenic and landfill gases: A comprehensive study.

The Science of the total environment·2024

Same author

Impact on air quality of carbon and sulfur volatile compounds emitted from hydrothermal discharges: The case study of Pisciarelli (Campi Flegrei, South Italy).

Chemosphere·2022

Same author

Dark Energy Survey Year 1 Results: Cosmological Constraints from Cluster Abundances, Weak Lensing, and Galaxy Correlations.

Physical review letters·2021

Hansenula saturnus respiration changes with culture age, showing sensitivity to antimycin A (AA) and salicylhydroxamic acid (SHAM). Mitochondrial protein synthesis, influenced by erythromycin, is crucial for specific respiratory pathways.

Area of Science:

* Microbiology
* Cell Biology
* Biochemistry

Background:

* Hansenula saturnus, a petite-negative yeast, exhibits dynamic respiratory patterns.
* Respiration is known to be influenced by culture age and specific inhibitors.
* Mitochondrial function and protein synthesis are critical cellular processes.

Purpose of the Study:

* To investigate the age-dependent respiratory variations in Hansenula saturnus.
* To elucidate the role of mitochondrial protein synthesis in regulating respiration.
* To characterize the sensitivity of different respiratory pathways to specific inhibitors.

Main Methods:

* Respiration assays were performed on yeast cultures at different growth phases.
* Sensitivity to antimycin A (AA) and salicylhydroxamic acid (SHAM) was assessed.

Related Experiment Videos

* The impact of erythromycin on mitochondrial protein synthesis and respiration was evaluated.

Main Results:

* Respiration sensitivity shifted from AA to AA + SHAM to SHAM as cultures aged.
* AA in the growth medium induced AA + SHAM-insensitive respiration, partially inhibited by azide.
* Erythromycin inhibited mitochondrial protein synthesis and prevented the induction of AA + SHAM-insensitive respiration, affecting cell viability.

Conclusions:

* Hansenula saturnus displays distinct, age-dependent respiratory activities.
* Mitochondrial protein synthesis is essential for the development of specific respiratory pathways in this yeast.
* These findings suggest a role for mitochondrial protein synthesis in the cell cycle of H. saturnus.