[Basic characteristics of Gunma clone-4 in the experimental study of metastasis]

Gunma clone-4 (Gc-4) cell was isolated by limiting dilution method from Radiological Science-Chiba- Toyama sarcoma which appeared spontaneously in a C3H/He mouse and cultured for generations. The character of Gc-4 cells was as follows: Gc-4 cells grew exponentially in vivo and in vitro, and the doubling time was 3.4 to 4.2 days in vivo and 20 to 22 hours in vitro respectively. Inoculation of 1 X 10(6) Gc-4 cells into A/Jackson mice and C57BL/6 mice resulted in rejection and disappearance within 17 days. Inoculation of 1 X 10(6) Gc-4 cells into C3H/He mice which were immunized with Gc-4 cells treated with 100 micrograms/ml of mitomycin C for 30 min. did not result in rejection. The Gc-4 cells showed histiocytic and fibroblastic characteristics morphologically and functionally, and corresponded to human malignant fibrous histiocytoma. Experiments on artificial and natural metastasis of Gc-4 cells revealed that Gc-4 cells had a high colony forming efficiency.