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Updated: Sep 29, 2026

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
Published on: August 23, 2013
Binding of plasma fibronectin to monolayer human endothelium
Abstract:
Interaction of exogenous plasma fibronectin with endothelium was examined using monolayer cultures of human umbilical vein endothelial cells. Plasma fibronectin was purified on gelatin-sepharose with 1 M arginine elution and iodinated with 125I by the solid phase glycoluril method. Endothelial monolayers were incubated with 0.5 to 15.0 mg/l (125I)fibronectin. Specificity of binding was 50 to 60% as determined by competition with 50-fold excess nonlabeled fibronectin. Binding reached maximal levels within 1 hour and without saturation over the concentration range. The bound glycoprotein showed minimal dissociation during subsequent incubation in media free of fibronectin.
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