Related Experiment Video
Updated: Aug 13, 2026
11:31
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
Published on: January 2, 2013
Summary
Weak red cell antibodies in sera are often anti-HLA B7, B17, or A28. Identifying these antibodies is crucial for accurate lymphocytotoxin testing and can be achieved using known HLA phenotypes of red cell panels.
Area of Science:
- Immunology
- Transfusion Medicine
- Human Leukocyte Antigen (HLA) Typing
Background:
- Weakly reacting antibodies in sera can present challenges in serological testing.
- Unidentified antibodies may interfere with accurate lymphocytotoxin testing.
Purpose of the Study:
- To identify the nature of weak, unidentified red cell antibodies found in sera undergoing HLA typing.
- To determine the prevalence of specific HLA antibodies among these sera.
Main Methods:
- A panel of 15 red cells was used to test 27 serum samples.
- A capillary tube antiglobulin technique, optimized for serum conservation, was employed.
- Results were correlated with known donor HLA phenotypes.
Main Results:
- 22 out of 27 sera (81%) contained definite or probable anti-HLA B7, B17, or A28 antibodies.
- Identified antibodies reacted specifically with red cells from donors with corresponding HLA types.
- Some sera showed broader reactivity, complicating definitive identification.
Conclusions:
- Weak unidentified red cell antibodies are frequently associated with specific HLA antibodies (B7, B17, A28).
- Utilizing red cell panels with known HLA phenotypes is essential for accurate antibody identification.
- This approach aids in resolving ambiguities in lymphocytotoxin testing.
Related Concept Videos
01:40Cell-mediated Immune Responses
Overview
00:42Cross-reactivity
Overview
02:45Blood Transfusion and Agglutination
Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
01:19Rh Blood Group
The Rhesus (Rh) antigen is crucial in determining blood groups and ensuring compatibility during blood transfusions.
01:10Blood Typing
Understanding an individual's blood group is a critical component of transfusion medicine. It ensures compatibility in blood transfusions, organ transplants, and even during pregnancy. Determining these blood groups involves the ABO and Rh blood typing systems, utilizing specific antigens and corresponding anti-sera to identify an individual's blood type.
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target antigens A,...
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target antigens A,...
01:01Hypersensitivity Reactions: Cytolytic Reactions
Type II hypersensitivity involves IgG and IgM antibodies targeting cell surface antigens, leading to cell destruction. This can occur through complement activation, antibody-dependent cell-mediated cytotoxicity (ADCC), or acting as opsonins for phagocytosis. When excessive, these reactions cause significant tissue damage.Drug-induced hemolytic anemia is a common example, where drugs like penicillin or cephalosporins bind to red blood cells, forming drug-protein complexes. These complexes...