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Updated: May 3, 2026

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Published on: December 30, 2014

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The adhesive interaction between polymorphonuclear leukocytes and endothelial cells in vitro

R L Hoover, R T Briggs, M J Karnovsky

    Cell
    |June 1, 1978
    PubMed

    Insights

    Polymorphonuclear neutrophils (PMN) show specific adhesion to endothelial cells, a key process in inflammation. This interaction is influenced by cell surface charge and chemotactic agents.

    Area of Science:

    • Cellular Biology
    • Immunology
    • Biomedical Research

    Background:

    • Polymorphonuclear neutrophils (PMN) are critical immune cells involved in acute inflammatory responses.
    • Endothelial cells form the inner lining of blood vessels and play a role in regulating immune cell trafficking.
    • Understanding PMN-endothelial cell interactions is vital for comprehending inflammatory processes in vivo.

    Purpose of the Study:

    • To investigate the adhesive specificity between polymorphonuclear neutrophils (PMN) and cultured endothelial cells.
    • To explore factors modulating PMN-endothelial cell adhesion, including divalent cations, neuraminidase, and chemotactic agents.
    • To establish a model system for studying PMN margination during acute inflammation.

    Main Methods:

    • Utilized a mono-layer collection assay to quantify PMN attachment to endothelial cells.
    • Employed direct cell counts via scanning electron microscopy for precise measurement of adhesion.
    • Assessed the impact of divalent cations, neuraminidase, and chemotactic agents on PMN-endothelial cell interaction.

    Main Results:

    • Demonstrated significant adhesive specificity of PMN for endothelial cells compared to other cell types.
    • Showed that divalent cations and neuraminidase modulate PMN-endothelial cell adhesion, suggesting a role for surface charge.
    • Observed an increase in PMN adherence to endothelial cells in the presence of chemotactic agents.

    Conclusions:

    • The study confirms specific adhesive interactions between PMN and endothelial cells.
    • Surface charge appears to play a role in PMN-endothelial cell adhesion, which can be modulated.
    • The developed system serves as a valuable model for in vivo studies of PMN margination in inflammation.

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