[Preparation of translating ribosomes by using a column with immobilized polyuridylic acid]

An improved method for preparation of translating ribosomes using columns with immobilized polyuridylic acid is described. A peculiarity of the method is that, first, optimal ratios of the components are used in the translation system for obtaining high yields of translating ribosomes. Second, purification of translating ribosomes from admixtures of non-translating particles is achieved by passing the buffer containing 5 mM MgCl2 and 250 mM NH4Cl through the column. The purity of the translating ribosomes is no less than 95%, the yield of active ribosomes is 5-20% of the initial amount of the ribosomes. Reagent expenditure is cut 15 to 20 times.