[Interaction of bifunctional binuclear platinum compounds with IMP and poly(I)]

A study has been made of the IMP or poly(I) interactions leading to substitution of X and Y ligands in binuclear platinum complexes of the general formula: [X-Pt(dien)]m+-(CH2)6-[(dien)Pt-Y]n+ (NO3)m+n, where dien stands for diethylenetriamine, X = H2O, Y = Br, m = 2, n = 1 (I), X = Y = H2O, m = n = 2 (II), and X = Y = Br, m = n = 1 (III). These compounds were found to be bifunctional reagents. Two reactive groupings of (I) bind to poly(I) at different rates due to the higher reactivity of [(dien)Pt(H2O)]2+ as compared to [(dien)PtBr]+. It allows to introduce the reactive bromotriaminoplatinum group into polynucleotide structure by rapid reaction with aquatriaminoplatinum group of compound (I). Increase in free bromide ion concentration in solution inhibits the reactivity of bromotriaminoplatinum groups, thus making possible regulation of the reactivity of this group introduced into a polynucleotide. The results obtained suggest that heterobifunctional platinum compounds can be used for design of complementary addressed reagents - oligo- and polynucleotides carrying the reactive groups.