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Related Experiment Videos

Histamine H-1 binding site on human polymorphonuclear leukocytes.

S Wescott, M Kaliner

    Inflammation
    |September 1, 1983
    PubMed
    Summary

    Human neutrophils (PMN) possess histamine H-1 receptors, crucial for allergic reactions. These receptors bind histamine, influencing the inflammatory response at allergic sites.

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    Area of Science:

    • Immunology
    • Allergy Research
    • Pharmacology

    Background:

    • Neutrophilic polymorphonuclear leukocytes (PMN) are key immune cells involved in allergic reactions.
    • Histamine is a primary mediator released during allergic responses.
    • Understanding histamine's interaction with PMNs is vital for allergy research.

    Purpose of the Study:

    • To characterize the binding of histamine to human neutrophils.
    • To identify the type and characteristics of histamine receptors on PMNs.

    Main Methods:

    • Utilized [3H]pyrilamine to study histamine binding to human PMNs.
    • Performed competitive binding assays with H-1 and H-2 antagonists.

    Main Results:

    • Human PMNs exhibit specific, saturable, and reversible binding of [3H]pyrilamine.
    • Binding demonstrated specificity for H-1 antagonists over histamine and H-2 antagonists.
    • Identified a homogeneous population of moderate-affinity H-1 receptors (Kd = 52 nM) on PMNs, numbering approximately 265,000 per cell.

    Conclusions:

    • Human PMNs possess functional histamine H-1 receptors.
    • These H-1 receptors on PMNs may play a significant role in mediating responses to histamine during allergic inflammation.

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