Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction between pectin and rat hindgut microflora.

A K Mallett, I R Rowland, A Wise

    Applied and Environmental Microbiology
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The changing landscape of antimicrobial resistance and use in South Africa: The need for access to new antibiotics: A position paper.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2024
    Same author

    Pregnancy after kidney and liver transplantation.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2024
    Same author

    Experience on postmortem minimally invasive tissue sampling to ascertain the cause of death determination in South African children: A case for implementing as standard of care.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2024
    Same author

    COVID-19 infection and maternal morbidity in critical care units in Scotland: a national cohort study.

    International journal of obstetric anesthesia·2022
    Same author

    Severe maternal morbidity in Scotland.

    Anaesthesia·2022
    Same author

    Structural features of subchondral bone cysts and adjacent tissues in hip osteoarthritis.

    Osteoarthritis and cartilage·2022
    Same journal

    A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.

    Applied and environmental microbiology·2026
    Same journal

    Sources and traits of bacteria and fungi found in the near-surface atmosphere.

    Applied and environmental microbiology·2026
    Same journal

    Advancing One Health in Africa through continental early warning environmental surveillance.

    Applied and environmental microbiology·2026
    Same journal

    Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

    Applied and environmental microbiology·2026
    Same journal

    Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

    Applied and environmental microbiology·2026
    Same journal

    Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

    Applied and environmental microbiology·2026
    See all related articles

    Dietary pectin significantly increases gut microbial nitrate reductase activity and luminal content in rats. This effect, linked to the insoluble fraction of gut microbes, is reversible and dependent on pectin, not pectic acid.

    Area of Science:

    • Gastroenterology
    • Microbiology
    • Nutritional Science

    Background:

    • Dietary fibers, like pectin, are known to influence gut microbiota and physiology.
    • Nitrate reductase activity in the gut is primarily microbial and can affect nitrogen metabolism.

    Purpose of the Study:

    • To investigate the effect of pectin supplementation on nitrate reductase activity and gut luminal content in rats.
    • To determine the microbial origin and characteristics of pectin-induced nitrate reductase activity.

    Main Methods:

    • Rats were fed a semisynthetic diet with or without pectin supplementation.
    • Gut contents were analyzed for nitrate reductase activity and luminal volume.
    • Antibiotic treatment (streptomycin, neomycin, bacitracin) was used to assess microbial involvement.

    Related Experiment Videos

  • Animals were switched between diets to observe reversibility.

    • Main Results:

    • Pectin supplementation increased nitrate reductase activity and luminal content in the rat intestine and cecum.
    • Nitrate reductase activity was localized to the insoluble fraction of gut contents and abolished by antibiotics.
    • The pectin-induced effects on cecal size and nitrate reduction were reversible upon diet change.
    • Pectic acid alone did not affect cecal size or microbial nitrate reductase activity.

    Conclusions:

    • Pectin significantly influences microbial metabolism in the rat alimentary tract, specifically enhancing nitrate reductase activity.
    • The observed effects are mediated by gut microbes and are dependent on pectin structure, not just its acidic form.