Cyst formation in metanephric organ culture induced by cis-dichlorodiammineplatinum (II)

A new experimental model of renal tubular cyst formation has been developed in metanephric organ culture. The addition of cis-dichlorodiammineplatinum (II), 50 micrograms/ml, to culture medium induces cystic changes during in vitro nephrogenesis. The model has particular utility in the study of basic mechanisms underlying renal tubular cystic changes, as well as the mechanisms by which nephrotoxins may mediate renal tubular injury.