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Sulphate transport in Candida utilis.

J A Benítez, A Alonso, J Delgado

    Folia Microbiologica
    |January 1, 1983
    PubMed
    Summary

    Sulphate uptake in Candida utilis is a regulated process, influenced by various sulphur compounds. This microbial sulphate transport is key to understanding sulphur amino acid biosynthesis.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Cell Biology

    Background:

    • Sulphate uptake is crucial for microbial growth and biosynthesis.
    • Understanding the regulation of nutrient transport is vital in microbial physiology.

    Purpose of the Study:

    • To elucidate the kinetics and regulatory mechanisms of sulphate uptake in Candida utilis.
    • To identify potential inhibitors and understand the role of endogenous sulphur compounds.

    Main Methods:

    • Enzyme kinetics analysis (Michaelis-Menten kinetics).
    • Investigating the effects of pH, temperature, and energy on sulphate transport.
    • Assessing the inhibitory effects of various compounds (molybdate, selenate, sulphite, etc.) on sulphate uptake.
    • Pre-incubation experiments to study endogenous inhibition.

    Main Results:

    • Sulphate uptake follows Michaelis-Menten kinetics with a Km of 1.43 mM.
    • The process is unidirectional and dependent on pH, temperature, and energy.
    • Competitive inhibition observed with molybdate, selenate, thiosulphate, chromate, and sulphite.
    • Mixed-type inhibition by dithionite.
    • Pre-incubation with various sulphur compounds significantly blocked sulphate uptake.
    • Endogenous inhibition by sulphate, sulphite, and thiosulphate was specific.

    Conclusions:

    • Extracellular sulphate incorporation in C. utilis is regulated by a heterogeneous pool of sulphur compounds.
    • These findings provide insights into the control mechanisms of sulphur amino acid biosynthesis in this yeast.

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