Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasminogen activator in differentiating mouse keratinocytes.

R R Isseroff, N E Fusenig, D B Rifkin

    The Journal of Investigative Dermatology
    |April 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Absence of LTBP-3 attenuates the aneurysmal phenotype but not spinal effects on the aorta in Marfan syndrome.

    Biomechanics and modeling in mechanobiology·2018
    Same author

    Acute exacerbation of carpal tunnel syndrome after Radiesse<sup>®</sup> injection for hand rejuvenation.

    The British journal of dermatology·2018
    Same author

    Short-term exposure of human sebocytes to 13-cis-retinoic acid induces acnegenic changes.

    The British journal of dermatology·2018
    Same author

    Corrigendum to "Systemic TAK-242 prevents intrathecal LPS evoked hyperalgesia in male, but not female mice and prevents delayed allodynia following intraplantar formalin in both male and female mice: The role of TLR4 in the evolution of a persistent pain state" [Brain Behav. Immun. 56 (2016) 271-280].

    Brain, behavior, and immunity·2018
    Same author

    Comparison of 10 murine models reveals a distinct biomechanical phenotype in thoracic aortic aneurysms.

    Journal of the Royal Society, Interface·2017
    Same author

    Deferoxamine: potential novel topical therapeutic for chronic wounds.

    The British journal of dermatology·2017

    Cellular differentiation in mouse keratinocytes is associated with increased intracellular levels of serine protease plasminogen activator (PA). This enzyme

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Dermatology

    Background:

    • Keratinocytes are essential skin cells that undergo differentiation to form the skin barrier.
    • Plasminogen activator (PA) is a serine protease with known roles in extracellular matrix remodeling.
    • The specific role of PA in keratinocyte differentiation remains largely undefined.

    Purpose of the Study:

    • To investigate the activity and localization of plasminogen activator (PA) during mouse keratinocyte differentiation.
    • To compare PA activity in primary and transformed keratinocyte cultures.
    • To determine the relationship between PA levels and the differentiation state of keratinocytes.

    Main Methods:

    • Measurement of plasminogen activator (PA) activity in cell lysates from primary and established mouse keratinocyte cultures.

    Related Experiment Videos

  • Assessment of morphologic differentiation and confluence in keratinocyte cultures.
  • Analysis of PA activity in relation to DNA synthesis and cell shedding.

    • Main Results:

    • PA activity was generally higher in transformed keratinocyte lines compared to primary cultures.
    • Cell-associated PA levels were highest in keratinocyte lines exhibiting significant morphologic differentiation.
    • PA activity increased as keratinocyte cultures reached confluence and showed signs of differentiation, peaking when DNA synthesis declined.
    • Highest specific PA activity was observed in shed cells, primarily cornified envelopes.

    Conclusions:

    • Intracellular plasminogen activator (PA) levels increase as keratinocytes differentiate.
    • The modulation of endogenous PA activity likely plays a significant role in keratinocyte maturation and terminal differentiation.
    • Further research is needed to fully elucidate the precise function of PA in these processes.