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Accumulation of altered viral nucleocapsids in mumps virus--persistently infected cell cultures
Archives of Virology
|January 1, 1983
Abstract:
Two long-term human cell cultures persistently infected with mumps virus accumulated increased amounts of morphologically altered viral nucleocapsids. Alterations involved size, heterogeneity, fine structure and shape. RNA present in intracellular nucleocapsids was predominantly of subgenomic size.
Insights
Persistent mumps virus infection in human cells led to altered viral nucleocapsid structures. Intracellular RNA within these altered nucleocapsids was mainly subgenomic in size.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Mumps virus is a significant human pathogen.
- Persistent viral infections can lead to cellular and viral alterations.
- Understanding viral replication and assembly is crucial for antiviral strategies.
Purpose of the Study:
- To investigate the long-term effects of persistent mumps virus infection on viral nucleocapsid morphology.
- To characterize the RNA content within altered nucleocapsids.
Main Methods:
- Long-term culture of human cells persistently infected with mumps virus.
- Electron microscopy to analyze viral nucleocapsid morphology.
- RNA analysis to determine the size of intracellular viral RNA.
Main Results:
- Accumulation of increased amounts of morphologically altered mumps virus nucleocapsids.
- Observed alterations included changes in size, heterogeneity, fine structure, and shape.
- Intracellular RNA within these nucleocapsids was predominantly of subgenomic size.
Conclusions:
- Persistent mumps virus infection induces significant morphological changes in viral nucleocapsids.
- The accumulation of subgenomic RNA within altered nucleocapsids suggests potential disruptions in viral replication or assembly processes.
- These findings may offer insights into the pathogenesis of persistent mumps virus infections.