Immuno chemical characterization of nitrate reductase from spinach leaves and roots

Nitrate reductase from spinach (Spinacia oleracea L.) leaves was purified to homogeneity and specific antibodies against the protein were raised by the immunization of rabbits. Using immunodiffusion and immunoprecipitation techniques, the enzymes from leaves and roots were compared. It was demonstrated that the two nitrate reductases were different proteins on the basis of their antigenic behaviour.