Studies on nitrogen and amino acid metabolism in hemodialysis patients using 15N-labelled compounds

The degree of 15N incorporation into serum albumin studied by 15N-urea administration in dialyzed patients on a 1.3 g/kg/day protein diet was shown to be almost the same as in non-dialyzed uremic patients on low protein diet, while there was no incorporation in a normal subject. 14.1 g of EAA and histidine was intravenously given in dialyzed patients on the high protein diet and improvements in the level of BUN and anemia were observed. The study with 15N-leucine in a patient proved that about 36% of EAA infused during dialysis was transferred into dialysate. AAD was prescribed by giving 15-20 g of EAA, histidine and tyrosine to patients at each dialysis. The AAD enabled us to give a large amount of EAA asymptomatically in a short time, improving anemia and decreasing the BUN level. 15N-glycine administration in a dialyzed patient proved that 15N incorporation into serum albumin was 3 times greater than his non-dialyzed uremic stage on a low protein diet, and that EAA serum concentrations and non-EAA which had not been added into dialysate were elevated.