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Related Experiment Videos

A rapid and highly sensitive solid-phase radioassay for plasminogen activators.

P C Roche, J D Campeau, S T Shaw

    Thrombosis Research
    |July 15, 1983
    PubMed
    Summary

    This study introduces a highly sensitive radioassay for measuring plasminogen activators, offering a significant improvement in detecting urokinase. This new method aids in assessing fibrinolytic potential for patients at risk of thromboembolic disease.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Analytical Chemistry

    Background:

    • Plasminogen activators are crucial in fibrinolysis.
    • Existing methods for measuring plasminogen activators have limitations in sensitivity and speed.
    • Thromboembolic diseases necessitate accurate monitoring of fibrinolytic activity.

    Purpose of the Study:

    • To develop a rapid and highly sensitive solid-phase radioassay for measuring plasminogen activators.
    • To enhance the detection limits for urokinase compared to existing techniques.
    • To evaluate the assay's utility in assessing fibrinolytic potential in clinical contexts.

    Main Methods:

    • Utilized a stable 125I-fibrinogen-latex bead product for a solid-phase radioassay.
    • Employed a radioisotopic technique for quantifying plasminogen activator activity.

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  • Tested the assay with euglobulin solutions from pre- and post-venous occlusion plasma.

    • Main Results:

    • The assay demonstrated high sensitivity, detecting 0.25 milli Ploug units/ml of urokinase.
    • Achieved a 100-fold increase in sensitivity over previous radioisotopic solid-phase methods.
    • Showed a 120-fold increase in sensitivity compared to the fibrin plate method.
    • Successfully detected plasminogen activator levels in plasma samples.

    Conclusions:

    • The developed radioassay is rapid, highly sensitive, and reproducible.
    • It offers a significant advancement in measuring plasminogen activators, particularly urokinase.
    • The assay is a valuable tool for monitoring fibrinolytic potential in patients susceptible to thromboembolic disorders.