Screening of hallucinogenic mushrooms with high-performance liquid chromatography and multiple detection

A rapid, sensitive and specific method for the screening of hallucinogenic mushrooms has been developed. High-performance liquid chromatography with simultaneous use of ultraviolet, fluorescence and electrochemical detection was employed. Separation of the mushroom components was achieved on a silica column using an alkaline aqueous methanolic eluent. The use of detector response ratios for identification of hallucinogenic indole alkaloids has been evaluated.