Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A computer electron microscope plotter for mapping spatial distributions in biological tissues.

R R Mize

    Journal of Neuroscience Methods
    |June 1, 1983
    PubMed
    Summary

    A new computer-based plotting system precisely maps organelle locations and analyzes their distribution in tissue specimens, aiding neurobiology research by detailing spatial arrangements of synapses and neurons.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development of the visual pathway is disrupted in mice with a targeted disruption of the calcium channel beta(3)-subunit gene.

    The Journal of comparative neurology·2001
    Same author

    Prenatal and postnatal expression of nitric oxide in the developing kitten superior colliculus revealed with NADPH diaphorase histochemistry.

    Visual neuroscience·2001
    Same author

    Nitric oxide, impulse activity, and neurotrophins in visual system development(1).

    Brain research·2000
    Same author

    Postnatal development of nitric oxide synthase expression in the mouse superior colliculus.

    The Journal of comparative neurology·2000
    Same author

    Normal development of the ipsilateral retinocollicular pathway and its disruption in double endothelial and neuronal nitric oxide synthase gene knockout mice.

    The Journal of comparative neurology·2000
    Same author

    Neuroscience training at the turn of the century: a summary report of the third annual ANDP survey.

    Nature neuroscience·2000

    Area of Science:

    • Neurobiology
    • Microscopy
    • Computational Biology

    Background:

    • Accurate spatial mapping of cellular structures is crucial for understanding tissue organization.
    • Existing methods may lack precision or automation for detailed distribution analysis.

    Purpose of the Study:

    • To develop and validate a computer-based plotting system for electron microscopy.
    • To enable precise mapping and quantitative analysis of organelle distribution in tissue specimens.

    Main Methods:

    • The system utilizes optical incremental shaft encoders and a microcomputer for data acquisition and analysis.
    • Software includes programs for tissue tracing, profile plotting, spatial analysis, and density calculations.
    • Data is processed using enhanced BASIC and commercial statistical software.

    Related Experiment Videos

    Main Results:

    • The system successfully maps organelle locations and analyzes their spatial distribution.
    • It calculates the depth and medial-lateral distance of profiles from tissue surfaces.
    • The Density program quantifies profile types, surface areas, and densities.

    Conclusions:

    • This computer-based plotting system offers a valuable tool for mapping cellular structures in electron microscopy.
    • It enhances the study of spatial distributions, particularly for synapses and neurons in the central nervous system.
    • The system has broad applicability in neurobiology and related research fields.