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Surface markers and function of circulating thyroid autoantibody-producing cells.

S Mariotti, G F del Prete, E Maggi

    The Journal of Clinical Endocrinology and Metabolism
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Foreword.

    Journal of endocrinological investigation·2012

    Pokeweed mitogen (PWM) significantly boosts in vitro thyroid autoantibody production by peripheral blood mononuclear cells (MNC) from patients with autoimmune thyroid disease. This PWM-stimulated synthesis is T-cell dependent, involving B lymphocytes with IgG and autoantigen receptors.

    Area of Science:

    • Immunology
    • Endocrinology
    • Autoimmunity

    Background:

    • Autoimmune thyroid diseases (AITD) are characterized by the production of autoantibodies against thyroid components.
    • The cellular mechanisms underlying autoantibody production in AITD require further elucidation.
    • Peripheral blood mononuclear cells (MNC) offer a model for studying in vitro immune responses in AITD.

    Purpose of the Study:

    • To investigate the in vitro synthesis of antithyroglobulin (anti-Tg) and antithyroid microsomal (anti-M) autoantibodies by MNC from AITD patients.
    • To determine the role of T lymphocytes and pokeweed mitogen (PWM) in stimulating autoantibody production.
    • To characterize the cell types involved in PWM-induced autoantibody synthesis.

    Main Methods:

    • Sensitive immunoradiometric assays were used to quantify anti-Tg and anti-M.

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  • MNC cultures were established from AITD patients and normal subjects, with and without PWM stimulation.
  • Cell depletion studies (T cells, Tg-binding cells) and T cell irradiation were performed to assess cellular contributions.

    • Main Results:

    • PWM significantly increased autoantibody concentrations and the number of positive cultures in AITD patients.
    • Autoantibody production was T-cell dependent, with T cells restoring PWM responsiveness in depleted cultures.
    • PWM-stimulated autoantibody synthesis involved B lymphocytes expressing surface IgG and DR antigens, with specificity for autoantigens.

    Conclusions:

    • In vitro synthesis of anti-Tg and anti-M can be frequently induced by PWM in patients with AITD.
    • PWM-stimulated thyroid autoantibody production is T-cell dependent and modulated by radiosensitive T lymphocytes.
    • The study identifies B lymphocytes with surface IgG and autoantigen receptors as key players in PWM-driven thyroid autoantibody synthesis.