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Techniques for Imaging Ca2+ Signaling in Human Sperm

Techniques for Imaging Ca²⁺ Signaling in Human Sperm

Published on: June 16, 2010

Cytochemical detection of hyaluronidase activity in single human and mouse sperm by an improved substrate-film

R Waibel, L C Ginsberg, G Ficsor

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society

|January 1, 1984

Abstract:

A substrate-film method is described that allows the detection of hyaluronidase activity in nearly 100% of single human and mouse sperm. The level of hyaluronidase activity as determined by halo diameters was greater in mouse than in human sperm. This simple method may have use as a screening method for identifying compounds that cause developmental or genetic defects in male germ cells, or for the diagnosis of infertility due to decreased hyaluronidase activity.

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Fluorimetric Techniques for the Assessment of Sperm Membranes

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Published on: November 28, 2018

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Medium-throughput Screening Assays for Assessment of Effects on Ca²⁺-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

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