Separation and identification of 3-methylcholanthrene-related compounds by gas chromatography-mass spectrometry

Separation and identification of 3-methylcholanthrene and its synthesized derivatives were examined by gas chromatography-mass spectrometry (GC-MS) system. Hydroxyl derivatives were silylated and all chemicals were applied to columns of OV-1 and Dexsil-300 at a constant temperature of 260 degrees and 290 degrees, respectively. Mass spectra of all derivatives were simple at ionization voltage of 20 eV and their molecular ions gave base peaks in most cases. Mass fragmentography plotted against molecular ions satisfactorily separated all standard derivatives so far examined.