The primary sequence of human pancreatic colipase

Area of Science:

• Biochemistry
• Molecular Biology
• Proteomics

Background:

• Colipase is an essential pancreatic cofactor for lipase activity.
• Understanding colipase structure is key to comprehending fat digestion mechanisms.

Purpose of the Study:

• To determine the complete amino acid sequence of human colipase.
• To compare the human colipase sequence with homologous proteins from other species.

Main Methods:

• Automated Edman degradation of purified human colipase.
• Cyanogen bromide (CNBr) peptide mapping.
• Sequence determination of porcine procolipase II.

Main Results:

• The complete 86-amino acid sequence of human colipase (human colipase86) was elucidated.
• Human colipase exhibits significant sequence homology with porcine, equine, and chicken colipases.
• Two previously missed residues in porcine procolipase II were identified.

Conclusions:

• The determined human colipase sequence provides a reference for comparative studies.
• High homology suggests conserved function and structure across species.
• Further research into human procolipase is warranted.