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Related Experiment Videos

Quantitative determination of complement components produced by purified hepatocytes.

G Ramadori, H Rasokat, R Burger

    Clinical and Experimental Immunology
    |January 1, 1984
    PubMed
    Summary

    Hepatocytes secrete significant amounts of complement proteins C3 and C5, suggesting they are the primary source of complement in plasma. This study quanties complement component secretion by liver cells.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • The liver is a major site of protein synthesis.
    • Complement system proteins are crucial for immune responses.
    • Hepatocytes are the primary functional cells of the liver.

    Purpose of the Study:

    • To quantitatively assess the secretion of complement components by hepatocytes.
    • To compare complement production in hepatocytes versus macrophages.
    • To investigate the role of hepatocytes as a source of plasma complement.

    Main Methods:

    • Isolation and primary culture of guinea-pig hepatocytes.
    • Quantification of complement C3 and C5 using ELISA.
    • Measurement of C2 and C4 functional activity via hemolytic assays.

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  • Assessment of cycloheximide's effect on complement production.

    • Main Results:

    • Hepatocytes secreted C3 at approximately 100 ng/10(6) cells/h and C5 at 15 ng/10(6) cells/24 h.
    • Complement secretion kinetics showed a plateau after 24 hours of culture.
    • Functional activity of C2 and C4 was dependent on culture medium exchange frequency.
    • Cycloheximide demonstrated reversible inhibition of complement production.

    Conclusions:

    • Guinea-pig hepatocytes synthesize substantial amounts of C3 and C5.
    • Hepatocytes are likely the predominant source of plasma complement components.
    • These findings support the hypothesis of hepatocytes being the major contributors to the complement system in circulation.