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Clonal tumorigenic endodermal cell lines producing basement membrane components.

E Engvall, R G Oshima, M J Brennan

    Experimental Cell Research
    |January 1, 1984
    PubMed
    Summary
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    Researchers developed highly malignant mouse tumors from endodermal cells. These tumors contain basement membrane proteins and retain original cell markers, useful for studying these components.

    Area of Science:

    • * Developmental Biology and Cancer Research

    Background:

    • * Mouse teratocarcinoma-derived endodermal cell line PF HR-9 was used.
    • * Long-term high-density cell culture preceded tumor induction.

    Purpose of the Study:

    • * To establish and characterize highly malignant tumor sublines from PF HR-9 cells.
    • * To investigate the composition of the extracellular matrix in induced tumors.
    • * To assess the retention of original cell markers in tumorigenic sublines.

    Main Methods:

    • * Subcutaneous injection of cultured PF HR-9 cells into syngeneic mice.
    • * Establishment and cloning of cell cultures from induced tumors.
    • * Analysis of tumor extracellular material for specific proteins and proteoglycans.
    • * Evaluation of cytoskeletal protein markers (Endo A and B) in cloned sublines.

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    Main Results:

    • * Cloned sublines derived from tumors were highly malignant in vivo.
    • * Tumor metastases were occasionally observed.
    • * Tumors exhibited abundant, laminated extracellular material containing type IV collagen, laminin, and heparan sulfate proteoglycan.
    • * Tumorigenic sublines retained cytoskeletal proteins Endo A and B, markers of the original PF HR-9 cells.

    Conclusions:

    • * The established malignant sublines are valuable models for studying basement membrane and cytoskeletal components.
    • * The tumors provide a source for isolating basement membrane macromolecules and their corresponding messenger RNAs (mRNAs).
    • * These cell lines and tumors offer a platform for biosynthetic studies on extracellular matrix and cytoskeletal proteins.