Modified fluid gelatin in leukapheresis accumulation and persistence in the body

Current methods for centrifuged granulocyte procurement involve the use of an agent to produce red cell rouleaux and enhance separation of leukocytes. Hydroxyethyl starch (HES), the agent most frequently used, has the disadvantage of causing progressive volume expansion and persisting in the circulation for long periods. We therefore assessed modified fluid gelatin (MFG) as a possible replacement for HES during granulocyte collection. We found that MFG is cleared more rapidly from the circulation with no traces remaining 7 days after multiple exposure, as determined by hydroxyproline measurement. However, after four consecutive daily infusions, we measured 0.60 liters plasma expansion in four individuals tested, somewhat lower than the 0.85 liters previously reported for HES. Modified fluid gelatin causes no impairment of coagulation with normal prothrombin time (PT), partial thromboplastin time (PTT), and platelet function.