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Related Experiment Videos

Bacterial-induced RBC alterations complicating necrotizing enterocolitis.

R W Novak

    American Journal of Diseases of Children (1960)
    |February 1, 1984
    PubMed
    Summary

    Bacterial enzymes can alter red blood cells (RBCs), making them vulnerable to antibodies. This was observed in infants with necrotizing enterocolitis, highlighting a risk associated with plasma transfusions.

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    Area of Science:

    • Microbiology
    • Immunology
    • Neonatal Medicine

    Background:

    • Bacterial enzymes can modify red blood cell (RBC) surfaces.
    • Altered RBCs become susceptible to antibody-mediated destruction.
    • Necrotizing enterocolitis (NEC) is a serious condition in infants.

    Purpose of the Study:

    • To investigate the role of bacterial enzymes in RBC alterations in infants with NEC.
    • To determine the association between RBC changes, bacterial isolates, and transfusion reactions.
    • To assess the efficacy of different plasma product types in preventing hemolysis.

    Main Methods:

    • Observational study of 20 infants with radiologically confirmed NEC.
    • Isolation and in vitro enzyme activity testing of bacterial species (Clostridium).
    • Clinical monitoring for hemolysis in relation to transfusion products (plasma-containing vs. washed products).

    Main Results:

    • RBC alterations were detected in 4 of 20 NEC cases, often severe (3/4 with bowel perforation).
    • Clostridium species were isolated from 3/4 affected infants and produced RBC-altering enzymes.
    • Two infants receiving plasma-containing products experienced hemolysis; those treated with washed products did not.

    Conclusions:

    • Bacterial enzymes, particularly from Clostridium, can cause RBC membrane alterations in NEC.
    • These alterations increase susceptibility to hemolysis, especially with plasma-containing transfusions.
    • Using washed products or plasma protein fractions without immunoglobulins may prevent transfusion-related hemolysis in NEC.

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