Neurite growth cone-substratum adherence increases in vitro

During experiments characterizing the turning response of dorsal root ganglion neurites toward NGF, it was observed that growth cone-substratum adherance increased with time in culture. The experiments reported here indicate that the observed increase in growth cone-substratum adherance is significant and can be detected with both collagen and poly-L-lysine substrates. The increased adherance is apparently due to a substance(s) produced and released by the ganglia which binds to the substrate, increasing adherance. Flow chamber studies indicate that the substrate-bound substance(s) may be necessary for neurite growth onto artificial tissue culture substrata.