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Effects of staphylococcal protein A on distribution of immune complexes in rats and uptake by phagocytic cells
Immune complexes (IC) of 125I-P30 anti-P30 when injected iv into normal BN rats were primarily sequestered in the spleen. Protein A (PA) when mixed with IC or when injected 6 hr before or after IC inhibited significantly the deposition of IC in the spleen. PA also inhibited deposition of IC in spleens of nude rats which lack thymus-dependent cells and functions. In vitro, PA enhanced significantly the uptake and catabolism of IC by PMN, but inhibited the uptake of IC by macrophages. This differential effect of PA on PMN and macrophages may partially explain the effect of PA on the deposition of IC in vivo.
Immune complexes (IC) of 125I-P30 anti-P30 when injected iv into normal BN rats were primarily sequestered in the spleen. Protein A (PA) when mixed with IC or when injected 6 hr before or after IC inhibited significantly the deposition of IC in the spleen. PA also inhibited deposition of IC in spleens of nude rats which lack thymus-dependent cells and functions. In vitro, PA enhanced significantly the uptake and catabolism of IC by PMN, but inhibited the uptake of IC by macrophages. This differential effect of PA on PMN and macrophages may partially explain the effect of PA on the deposition of IC in vivo.