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Chromosomal locus for staphylococcal enterotoxin B.

W M Shafer, J J Iandolo

    Infection and Immunity
    |April 1, 1978
    PubMed
    Summary

    The staphylococcal enterotoxin B (SEB) gene in food-poisoning Staphylococcus aureus strains is likely chromosomal, not plasmid-encoded. Experiments showed SEB production was independent of identified plasmids carrying resistance genes.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Food Safety

    Background:

    • Staphylococcus aureus is a significant cause of foodborne illness.
    • Staphylococcal enterotoxin B (SEB) is a key virulence factor.
    • The genetic basis of SEB production, whether chromosomal or plasmid-mediated, requires clarification.

    Purpose of the Study:

    • To determine the genetic location of the staphylococcal enterotoxin B (SEB) gene in food-poisoning isolates of Staphylococcus aureus.
    • To investigate the role of plasmids in SEB production and antibiotic resistance.

    Main Methods:

    • Analysis of bacterial isolates (strains S6 and 277) using neutral sucrose gradient centrifugation.
    • Plasmid characterization, including elimination and transduction experiments.
    • Assessment of SEB production and antibiotic resistance (cadmium, penicillin, tetracycline) in parent strains and derivatives.

    Main Results:

    • Strain S6 possessed a 37S plasmid conferring cadmium resistance, but SEB production was independent of this plasmid.
    • Strain 277 harbored a 37S penicillin resistance plasmid and a 21S tetracycline resistance plasmid; SEB production was unaffected by the 37S plasmid.
    • Introduction of the 21S tetracycline resistance plasmid did not confer SEB production in a non-toxigenic strain.

    Conclusions:

    • The staphylococcal enterotoxin B (SEB) gene(s) in the investigated food-poisoning Staphylococcus aureus isolates are located on the chromosome.
    • Plasmids associated with antibiotic resistance in these strains are not responsible for SEB enterotoxin production.

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