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Intermediates in 16-androstene biosynthesis in neonatal porcine testis
FEBS Letters
|February 13, 1984
Abstract:
The metabolism of pregnenolone has been studied in microsomal preparations of testes from 3-week old piglets. Metabolites identified after derivatization by capillary gas-chromatography and further by combined gas chromatography-mass spectrometry were: 17-hydroxy- and 16 alpha-hydroxypregnenolone, dehydroepiandrosterone, 5-androstenediols, 5-pregnenediol and andien-beta. Pregnenolone and 16-dehydro-pregnenolone, previously not separable by other methods, were successfully resolved as methyl oximetrimethylsilyl ethers using capillary column gas-chromatography.