The effect of heating on Y-chromosome detection

Related Experiment Video

Updated: Oct 11, 2026

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation
09:28

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation

Published on: August 7, 2010

M Tomita, I Ijiri, K Shimosato

    Forensic Science International
    |January 1, 1984
    PubMed

    The effect of heating on Y-chromosome detection was investigated. Heat-treated blood was divided into four groups according to the hemolysis pattern obtained by the coil planet centrifuge system. A significant difference between the Y-positive nuclei of males and females was observed up to group III of the hemolysis pattern, and it was possible to determine the sex of the blood donor in spite of karyolysis and degeneration of blood components. However, sex determination of blood in group IV, indicating complete hemolysis, was impossible because of overlapping between male and female Y-chromosome counts. Practical application of this sex determination method was successful even with severely burned cadavers. However, it was suggested that putrefaction together with heat damage made the identification nearly impossible.

    More Related Videos

    Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae09:40

    Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

    Published on: September 23, 2011

    Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells15:54

    Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

    Published on: June 14, 2014

    Related Concept Videos

    Karyotyping01:17

    Karyotyping

    Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
    X and Y Chromosomes02:32

    X and Y Chromosomes

    Among mammals, the gender of an organism is determined by the sex chromosomes. Humans have two sex chromosomes, X and Y. Every human diploid cell has 22 pairs of autosomes and one pair of sex chromosomes. A human female has two X chromosomes, while a male has one X chromosome and one Y chromosome.
    The germline cells such as egg and sperm cells carry only half the number of chromosomes, i.e., 22 autosomes and one sex chromosome. All eggs have an X chromosome, while sperm cells can carry an X or...
    The Y Chromosome Determines Maleness02:19

    The Y Chromosome Determines Maleness

    The Y chromosome is a sex chromosome found in several vertebrates and mammals, including humans. In addition to 22 pairs of autosomes, the human males have one X chromosome and one Y chromosome. In these organisms, the presence or absence of the Y chromosome determines the development of male traits.
    Evolution
    Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size. Today,...