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Modified human fibrinogen labeled with 111In
The International Journal of Applied Radiation and Isotopes
|January 1, 1984
Summary
Researchers developed a new method to label human fibrinogen with Indium-111. This stable, clottable labeled protein shows promise for future medical imaging applications.
Area of Science:
- Biochemistry
- Radiochemistry
- Medical Imaging
Background:
- Fibrinogen is a key protein in blood clotting.
- Radiolabeling proteins is crucial for diagnostic imaging.
- Developing stable and effective radiolabeled tracers is an ongoing challenge.
Purpose of the Study:
- To report a novel method for labeling human fibrinogen with Indium-111.
- To assess the stability and functionality of the labeled fibrinogen.
- To explore the potential of this tracer in imaging studies.
Main Methods:
- Covalent attachment of the cyclic anhydride of Diethylenetriaminepentaacetic acid (DTPA) to human fibrinogen.
- Labeling the modified fibrinogen with Indium-111 acetate.
- Assessing the clottability and in vitro stability of the radiolabeled product.
Main Results:
- The labeling method achieved an average yield of 90 +/- 3%.
- The resulting Indium-111 labeled fibrinogen was highly clottable.
- The labeled fibrinogen demonstrated excellent in vitro stability.
Conclusions:
- A robust and efficient method for radiolabeling human fibrinogen with Indium-111 has been established.
- The labeled fibrinogen retains its biological function and stability.
- This Indium-111 labeled fibrinogen holds significant potential for diagnostic imaging applications.