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Genetic complementation between natural strains of red clover mottle virus
Intervirology
|January 1, 1978
Summary
Red clover mottle virus infectivity is determined by its middle component, which influences symptom development in host plants. Top component formation is also affected by interactions between the middle and bottom components.
Area of Science:
- Plant virology
- Molecular plant pathology
Background:
- Red clover mottle virus (RCMV) is a plant pathogenic virus.
- Understanding viral component roles is crucial for disease management.
Purpose of the Study:
- To elucidate the roles of RCMV components in infectivity and symptom expression.
- To investigate the interactions between RCMV components.
Main Methods:
- Purification of three RCMV strains.
- Separation of viral components using density gradient and isopycnic centrifugation.
- Infectivity assays on Phaseolus vulgaris, Pisum sativum, and Chenopodium quinoa.
Main Results:
- Infectivity enhancement observed in mixtures of middle and bottom components.
- Symptoms on Pisum sativum and Chenopodium quinoa are determined by the middle component.
- Top component formation is influenced by both middle and bottom components.
Conclusions:
- The middle component of RCMV dictates symptom development in host plants.
- Interactions between RCMV components are essential for viral replication and assembly.