Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

P1 plasmid replication: replicon structure.

A L Abeles, K M Snyder, D K Chattoraj

    Journal of Molecular Biology
    |March 5, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Regulation of human glioma cell migration, tumor growth, and stemness gene expression using a Lck targeted inhibitor.

    Oncogene·2018
    Same author

    Interaction of water vapour with twenty different poly-L-amino acids and their excess hydration in presence of sodium chloride.

    Indian journal of biochemistry & biophysics·2012
    Same author

    Surface activity coefficients of spread monolayers of behenic acid salts at air-water interface.

    Advances in colloid and interface science·2006
    Same author

    Thermodynamics of the interaction of globular proteins with powdered stearic acid in acid pH.

    Biomacromolecules·2006
    Same author

    A generalized scale of free energy of excess adsorption of solute and absolute composition of the interfacial phase.

    Langmuir : the ACS journal of surfaces and colloids·2005
    Same author

    Adsorption of biopolymers at hydrophilic cellulose-water interface.

    Biopolymers·2005
    Same journal

    UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

    Journal of molecular biology·2026
    Same journal

    Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

    Journal of molecular biology·2026
    Same journal

    Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

    Journal of molecular biology·2026
    Same journal

    UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

    Journal of molecular biology·2026
    Same journal

    Structural basis for the pro-amyloidogenic action and ligand binding of a novel W72R variant of human apolipoprotein A-I.

    Journal of molecular biology·2026
    Same journal

    Cryo-EM Structure of the C. Elegans Septin Tetramer Reveals a Revised Architecture and Conserved Positional Orthology.

    Journal of molecular biology·2026
    See all related articles

    Bacteriophage P1 plasmid replication involves specific DNA elements, including the repA gene and repeat sequences (incA, incC), which control its unit-copy maintenance in Escherichia coli. These elements share organizational similarities with the F plasmid.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Bacteriophage P1 establishes lysogeny in Escherichia coli by existing as a unit-copy plasmid.
    • Understanding the molecular mechanisms of P1 plasmid maintenance is crucial for comprehending its lifecycle.

    Purpose of the Study:

    • To identify and characterize the plasmid-encoded elements responsible for P1 plasmid maintenance.
    • To investigate the role of specific DNA sequences and proteins in P1 replication control.

    Main Methods:

    • DNA fragment cloning and functional analysis to determine replication capacity.
    • DNA sequencing to identify open reading frames and repeat sequences.
    • Genetic identification of the repA gene.
    • Incompatibility assays using cloned repeat sequences in pBR322.

    Related Experiment Videos

    Main Results:

    • A 2081 base-pair fragment confers controlled replication ability to the P1 plasmid.
    • The repA gene encodes a 32,000 Mr protein essential for P1 replication.
    • Upstream (incC) and downstream (incA) repeat sequences are involved in P1 replicon incompatibility.
    • P1 plasmid maintenance regions show organizational similarity to the F plasmid, despite low DNA homology.

    Conclusions:

    • The repA gene and incA/incC repeat regions are key elements for P1 plasmid maintenance.
    • The identified repeat sequences play a regulatory role in plasmid replication, potentially interacting with host factors like dnaA.
    • The conserved organization of maintenance regions in P1 and F plasmids suggests common evolutionary or functional principles.