Determination of apoprotein B in the skin

To determine the origin of cutaneous cholesterol a method has been developed for extracting and determining by electro-immuno-assay apoprotein B in the skin as already performed by Hoff at the arterial level. This study demonstrated the presence of apoprotein B in the skin and its quantification. The easy and reliable technique employed required only conventional laboratory instruments. Repeatability and reproducibility studies give encouraging results comparable with those obtained on arterial tissue. They show that at least one part of skin cholesterol originates in low density lipoproteins.