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Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
Published on: September 7, 2018
Syngeneic sensitization of mouse lymphocytes on monolayers of thyroid epithelial cells. VI. Evaluation of its
The determination of the frequency of normal spleen cell involvement in Primary syngeneic sensitization (PSS) against thyroid epithelial cells (TEC) was performed using the limiting dilution analysis. It was found that their frequency, which reaches 1:5440, is related to the expression of major histocompatibility complex (MHC) antigens (K end or D end) and of the specific antigen thyroglobulin (Tg) exposed on the surface of the TEC monolayer. Moreover, a significant diminution of this frequency was obtained by the addition of 25% normal syngeneic lymph node (LN) cells.
The determination of the frequency of normal spleen cell involvement in Primary syngeneic sensitization (PSS) against thyroid epithelial cells (TEC) was performed using the limiting dilution analysis. It was found that their frequency, which reaches 1:5440, is related to the expression of major histocompatibility complex (MHC) antigens (K end or D end) and of the specific antigen thyroglobulin (Tg) exposed on the surface of the TEC monolayer. Moreover, a significant diminution of this frequency was obtained by the addition of 25% normal syngeneic lymph node (LN) cells.
