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Electronic device for microelectrode recordings in epithelial cells.

J F Garcia-Diaz, S Stump, W M Armstrong

The American Journal of Physiology

|March 1, 1984

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A new device enables continuous electrophysiological measurements in epithelial tissues. It accurately assesses membrane integrity and microelectrode placement, crucial for reliable research data.

Area of Science:

Electrophysiology
Biophysics
Cell Biology

Background:

Accurate electrophysiological measurements in epithelial tissues are vital for understanding cellular function.
Distinguishing true cellular signals from artifact is a persistent challenge in microelectrode recordings.
Existing methods may not provide continuous, real-time assessment of measurement quality.

Purpose of the Study:

To describe a novel device for continuous, open-circuit electrophysiological measurements in epithelial tissues.
To enable simultaneous monitoring of transepithelial potential and microelectrode potential.
To provide real-time indices of membrane integrity and microelectrode impalement quality.

Main Methods:

Direct measurement of transepithelial potential (VT) and microelectrode potential (VM).

Related Experiment Videos

Application of transepithelial current pulses for resistance (RT) and VM/VT ratio monitoring.

Continuous recording of microelectrode resistance (RM) using current pulses.

Compensation for solution IR drop and use of a high-impedance electrometer with stray capacitance neutralization.

Main Results:

The device allows continuous monitoring of VT and VM in open-circuit conditions.
Transepithelial resistance (RT) and the VM/VT ratio provide insights into membrane status and impalement.
Microelectrode resistance (RM) monitoring distinguishes acceptable impalements from tip artifacts.
The system effectively compensates for solution resistance and electrometer limitations.

Conclusions:

This device offers a significant advancement for continuous, artifact-aware electrophysiological studies of epithelia.
It enhances the reliability of microelectrode impalements and the interpretation of electrophysiological data.
The technology is valuable for assessing epithelial barrier function and cellular electrophysiology with improved accuracy.