[Intraperitoneal transplantation derived from spontaneous colonic cancer of WF rat strain]

Intraperitoneal transplantation of spontaneous colon carcinoma of WF strain has been established. There are two lines of transplantable colon carcinoma derived from the male and female rat of the same litter (MM4-48H). These two lines are now (Sept. '83) at the 16th generation. They have been succeeded every 3 weeks intraperitoneally using 4-weeks old male and female siblings of WF strain. Histology of these transplantable colon carcinoma was well-differentiated tubular adenocarcinoma at the first few generations but changed gradually medullary tubular pattern at the 16th generation. We believe this is the first report of transplantable tumor lines from the spontaneous colon carcinoma itself and these lines can be used for screening test of carcinostatics.