Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vero microcultures for adenovirus neutralization tests.

J C Hierholzer, P G Bingham

    Journal of Clinical Microbiology
    |June 1, 1978
    PubMed
    Summary

    A new microculture neutralization test effectively measures human adenovirus antibody levels. This simplified assay in Vero cells provides reliable serum titers comparable to traditional methods.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively.

    Journal of clinical microbiology·1999
    Same author

    Adenovirus types 2, 8, and 37 associated with genital infections in patients attending a sexually transmitted disease clinic.

    Journal of clinical microbiology·1995
    Same author

    Comparison of monoclonal biotin-avidin enzyme immunoassay and monoclonal time-resolved fluoroimmunoassay in detection of respiratory virus antigens.

    Clinical and diagnostic virology·1995
    Same author

    Neonatal pulmonary insufficiency caused by adenovirus infection successfully treated with extracorporeal membrane oxygenation.

    The Journal of pediatrics·1994
    Same author

    Antigen detection in human respiratory Coronavirus infections by monoclonal time-resolved fluoroimmunoassay.

    Clinical and diagnostic virology·1994
    Same author

    Subgrouping of respiratory syncytial virus strains from Australia and Papua New Guinea by biological and antigenic characteristics.

    Archives of virology·1994

    Area of Science:

    • Virology
    • Immunology
    • Cell Biology

    Background:

    • Human adenoviruses are a significant cause of human illness.
    • Accurate measurement of adenovirus-specific antibodies is crucial for understanding immunity and vaccine efficacy.
    • Existing neutralization tests can be labor-intensive and require specialized cell cultures.

    Purpose of the Study:

    • To develop and validate a simplified microculture neutralization test for quantifying antibodies against 35 human adenovirus serotypes.
    • To assess the reliability and comparability of the new test with established methods.

    Main Methods:

    • A microculture neutralization test was established using Vero cells.
    • The test involves staining uninfected cells with crystal violet and macroscopic observation at 5 days.
    • Serum titers were determined by measuring the inhibition of adenovirus-induced cell toxicity.

    Main Results:

    • The Vero microculture neutralization test accurately measured specific antibody levels to all 35 human adenovirus serotypes.
    • Serum titers obtained were comparable to those from traditional tube macroculture methods using monkey kidney, human embryonic kidney, and Vero cells.
    • The test demonstrated adaptability of human adenoviruses in Vero cells, replicating without helper viruses.

    Conclusions:

    • The described microculture neutralization test offers a simplified and efficient method for measuring human adenovirus-specific antibodies.
    • This assay provides a reliable alternative to conventional methods, facilitating broader serological studies.
    • The findings support the utility of Vero cells for adenovirus neutralization assays.

    Related Experiment Videos