Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium-dependent calmodulin binding to cholinergic synaptic vesicles.

J E Hooper, R B Kelley

    The Journal of Biological Chemistry
    |January 10, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Refilling Prescriptions.

    Atlanta journal-record of medicine·2022
    Same author

    Matched filters for bin picking.

    IEEE transactions on pattern analysis and machine intelligence·2012
    Same author

    Error analysis of surface normals determined by radiometry.

    IEEE transactions on pattern analysis and machine intelligence·2011
    Same author

    The complete prosthesis and disorders of the temporomaxillary joint.

    L' Information dentaire·2010
    Same author

    User satisfaction with rehabilitation services delivered using Internet video.

    Journal of telemedicine and telecare·2003
    Same author

    Estrogen agonist and antagonist action on the human estrogen receptor in Drosophila.

    Endocrinology·2000
    Same journal

    Wanted and unwanted modifications of mRNA, and their effect on gene expression and signaling.

    The Journal of biological chemistry·2026
    Same journal

    TGF-β2 drives lipid droplet accumulation in chondrocytes through the TβRI/p-Smad3/Fabp5 axis.

    The Journal of biological chemistry·2026
    Same journal

    Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivo.

    The Journal of biological chemistry·2026
    Same journal

    Substrate and target selectivity of 4'-fluoroadenosine against viral and host polymerases.

    The Journal of biological chemistry·2026
    Same journal

    Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

    The Journal of biological chemistry·2026
    Same journal

    Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

    The Journal of biological chemistry·2026
    See all related articles

    Calmodulin binds to cholinergic synaptic vesicles in a calcium-dependent manner, indicating a role in nerve terminal function. This binding is specific and saturable, suggesting a key regulatory mechanism.

    Area of Science:

    • Neuroscience
    • Molecular Biology
    • Biochemistry

    Background:

    • Calmodulin is a critical calcium-binding protein involved in numerous cellular processes.
    • Calcium-dependent calmodulin binding has been observed in various secretory preparations.

    Purpose of the Study:

    • To investigate the presence and characteristics of calmodulin-binding sites on cholinergic synaptic vesicles.
    • To determine the affinity, specificity, and orientation of calmodulin binding to these vesicles.

    Main Methods:

    • Calcium-dependent calmodulin binding assays using isolated cholinergic synaptic vesicles.
    • Characterization of binding kinetics, affinity (KD), and inhibition by trifluoperazine.
    • Assessment of binding specificity against troponin C.
    • Analysis of calmodulin-binding proteins via SDS-PAGE.

    Related Experiment Videos

    Main Results:

    • A high-affinity, saturable calmodulin-binding site (KD = 10 nM) was identified on cholinergic synaptic vesicles.
    • Binding is calcium-dependent, inhibited by trifluoperazine, and highly specific for calmodulin.
    • The binding site is cytoplasmically oriented on intact vesicles.
    • A subset of nerve terminal calmodulin-binding proteins are localized to synaptic vesicles.

    Conclusions:

    • Cholinergic synaptic vesicles possess a specific, high-affinity calmodulin-binding site.
    • This interaction is likely involved in calcium-dependent regulation of synaptic vesicle function.
    • Calmodulin may play a direct role in modulating neurotransmitter release at the synapse.