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A specific indicator for mouse lymphocyte C3b receptors.

W D Linscott, R Ranken

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 1, 1978
    PubMed
    Summary

    Sheep red blood cells coated with guinea pig complement proteins (EAC43bgp) effectively detect mouse C3b receptors. Unlike mouse C3b, degradation to C3d on these cells does not affect binding, ensuring specific detection of C3b receptors.

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    Area of Science:

    • Immunology
    • Complement System
    • Cellular Receptors

    Background:

    • Complement component C3b plays a crucial role in immune responses.
    • Understanding C3b receptor interactions is vital for immunological studies.
    • Existing methods for detecting C3b receptors can be subject to interference from C3d.

    Purpose of the Study:

    • To develop a sensitive and specific indicator for mouse lymphocyte C3b receptors.
    • To evaluate the utility of sheep erythrocytes sensitized with guinea pig complement (EAC43bgp) for this purpose.

    Main Methods:

    • Preparation of IgM-sensitized sheep erythrocytes bearing guinea pig C4 and C3b (EAC43bgp).
    • Assessing the binding of EAC43bgp to mouse spleen cells.
    • Investigating the effect of C3b degradation to C3d on binding affinity to mouse and guinea pig spleen cells.

    Main Results:

    • EAC43bgp exhibited strong reactivity with mouse spleen cell C3b receptors, comparable to optimally prepared EACmo.
    • Conversion of C3b to C3d on EAC43bgp resulted in complete loss of reactivity with mouse spleen cells.
    • Binding to guinea pig spleen cells remained high even after C3b degradation.

    Conclusions:

    • EAC43bgp serve as a sensitive and specific indicator for mouse lymphocyte C3b receptors.
    • The differential binding after C3b degradation prevents misleading results from C3d receptors.
    • This method offers an advantage over cells bearing mouse C3b, which can bind to C3d receptors.

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