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Related Experiment Videos

Dynamics and equilibria of nucleosomes at elevated ionic strength.

T D Yager, K E van Holde

    The Journal of Biological Chemistry
    |April 10, 1984
    PubMed
    Summary
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    Chicken erythrocyte nucleosomes with long DNA dissociate in salt, releasing free DNA. This salt-induced dissociation is reversible and increases with salt concentration, suggesting a mass action mechanism for nucleosome stability.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Chromatin Structure

    Background:

    • Nucleosomes are the fundamental units of DNA packaging in eukaryotes.
    • Understanding nucleosome stability is crucial for comprehending DNA accessibility and gene regulation.

    Purpose of the Study:

    • To investigate the stability of chicken erythrocyte nucleosomes with long DNA in varying salt concentrations.
    • To elucidate the mechanism of salt-induced nucleosome dissociation and its reversibility.

    Main Methods:

    • Preparation of chicken erythrocyte nucleosomes with defined long DNA lengths.
    • Analysis of nucleosome stability using boundary and band sedimentation.
    • Assessment of dissociation and structural changes via particle gel electrophoresis.

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    Main Results:

    • Salt treatment slowly generates a species identified as free DNA, indicating nucleosome dissociation.
    • Nucleosome dissociation is reversible and follows the law of mass action, enhanced by dilution.
    • Increased salt concentration promotes dissociation, and intact nucleosomes exhibit a reversible decrease in sedimentation coefficient.

    Conclusions:

    • Salt induces nucleosome dissociation independent of non-core proteins or shorter DNA lengths.
    • The observed structural changes in intact nucleosomes suggest DNA unfolding or terminal region unbinding.
    • Nucleosome stability is sensitive to salt concentration, impacting chromatin structure and dynamics.