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Related Experiment Videos

Three-dimensional structure of a membrane-microtubule complex.

J M Murray

    The Journal of Cell Biology
    |January 1, 1984
    PubMed
    Summary

    The unicellular algae Distigma proteus has a unique membrane-microtubule complex. This structure, featuring a crystalline particle array, may scaffold microtubule attachment, revealed by 3D reconstruction.

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    Area of Science:

    • Cell Biology
    • Microscopy
    • Biophysics

    Background:

    • The unicellular alga Distigma proteus possesses an unusual association between its cell membrane and aligned microtubules.
    • This membrane-microtubule complex is stable and can be isolated.
    • The complex includes a crystalline array of membrane particles.

    Purpose of the Study:

    • To investigate the structure of the membrane-microtubule complex in Distigma proteus.
    • To determine the relationship between the membrane particle array and microtubule association.
    • To generate a high-resolution 3D model of the membrane array.

    Main Methods:

    • Isolation of membrane-microtubule complexes from Distigma proteus.
    • Radioiodine labeling of cell surface peptides.
    • Electron microscopy and Fourier transform techniques for 3D reconstruction.
    • Analysis of lattice spacings and correlations.

    Main Results:

    • A 3D model of the membrane array was calculated at 2.5 nm resolution.
    • The major peptide component of the array was identified.
    • Similarities in lattice spacings between the membrane array and microtubules were observed.
    • Evidence suggests the membrane particle array acts as scaffolding for microtubule attachment.

    Conclusions:

    • The membrane-microtubule complex in Distigma proteus is a well-ordered structure.
    • The crystalline membrane particle array likely plays a crucial role in anchoring microtubules to the cell membrane.
    • This finding provides insights into the mechanisms of cytoskeletal-membrane interactions.

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