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Updated: Sep 21, 2026

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
05:39

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Published on: November 7, 2010

Radioimmunoassay of a glucose-containing tetrasaccharide using a monoclonal antibody

A Lundblad, K Schroer, D Zopf

    Journal of Immunological Methods
    |March 30, 1984
    PubMed

    Monoclonal antibodies were produced in BALB/cJ mice against a urinary glucose-containing tetrasaccharide (Glc)4 coupled to keyhole limpet hemocyanin (KLH). The immune response was studied using 7 different strains of mice and 11 different immunization protocols. From 6 fusions using the Sp2/0 myeloma cell line 2 hybrids were obtained producing antibodies (subtype IgG2b and IgG3, respectively) that were capable of binding reduced tetrasaccharide. Specificity studies showed that one of these antibodies (61.1) was useful for radioimmunoassay of (Glc)4.

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